Roche NimbleGen's Revolutionary Sequence Capture Technology Now Available for Use in Research LaboratoriesThursday September 4 10:00 PMAt the High-Throughput Sequencing Solutions lab of the University of Washington in Seattle, Sequence Capture 385K Custom Delivery Arrays are already in full use - with very good success. "We found NimbleGen Sequence Capture Arrays and protocol very easy to use and routinely observed successful capture experiments," says Michael Dorschner, Director of the unit. He also appreciates the quality control that comes with the system. "It not only provides robust equipment for the elution of target-specific templates but also a qPCR-based assay to evaluate the degree of enrichment." Besides Sequence Capture Delivery Arrays, Roche also provides validated equipment and kits, including a new instrument called Elution Station, to facilitate researchers to achieve consistent and reproducible capture results. Moreover, the cost savings of performing such cutting-edge research using NimbleGen Sequence Capture Arrays is a big advantage when compared with previous techniques. As Dorschner explains, the "Roche NimbleGen's Sequence Capture Technology provides a cost-effective alternative to PCR for the enrichment of genomic targets facilitating large-scale resequencing projects and the development of a multitude of next-generation sequencing-based assays." Not only does the use of Sequence Capture Arrays tackle a major bottleneck in DNA sequencing system, the isolation of target genomic regions, but it also enhances the advantages of next-generation sequencing. The arrays are a perfect fit for the long reads of the 454 sequencing technology and thus enable haplotyping and identification of variations such as insertions, deletions, and SNPs down to single base pairs. Currently, captured DNA fragments can be directly utilized in the 454 system library preparation process as input for sequencing. "The Roche NimbleGen Sequence Capture System has revolutionized the way genomic regions of interest can be sequenced," says Lynne Nazareth, Associate Professor at the Human Genome Sequencing Center at Baylor College of Medicine, Houston. "The system provides the scientific community with an invaluable tool for the rapid interrogation of genetic sequences, especially when combined with the next-generation sequencing technologies. We have successfully used the capture technology to enrich for targeted genes and found the system to be user-friendly and cost-effective, thereby facilitating our resequencing projects." Roche NimbleGen is currently developing protocols that will allow the direct addition of 454 adapters into the Sequence Capture workflow, thereby further streamlining the workflow and facilitate even faster turnaround time. The goal is to provide researchers with a more complete solution for targeted next-generation sequencing - in their own lab, on their own bench, on their own schedule. Roche NimbleGen is a leading innovator, manufacturer and supplier of a proprietary suite of DNA microarrays, consumables, instruments and services. Roche NimbleGen uniquely produces high-density arrays of long oligo probes that provide greater information content and higher data quality necessary for studying the full diversity of genomic and epigenomic variation. The improved performance is made possible by Roche NimbleGen's proprietary Maskless Array Synthesis (MAS) technology, which uses digital light processing and rapid, high-yield photochemistry to synthesize long oligo, high-density DNA microarrays with extreme flexibility. For more information about Roche NimbleGen, please visit the company's website at www.nimblegen.com. About Roche Headquartered in Basel, Switzerland, Roche is one of the world's leading research-focused healthcare groups in the fields of pharmaceuticals and diagnostics. As the world's biggest biotech company and an innovator of products and services for the early detection, prevention, diagnosis and treatment of diseases, the Group contributes on a broad range of fronts to improving people's health and quality of life. Roche is the world leader in in-vitro diagnostics and drugs for cancer and transplantation, and is a market leader in virology. It is also active in other major therapeutic areas such as autoimmune diseases, inflammatory and metabolic disorders and diseases of the central nervous system. In 2007 sales by the Pharmaceuticals Division totalled 36.8 billion Swiss francs, and the Diagnostics Division posted sales of 9.3 billion francs. Roche has R&D agreements and strategic alliances with numerous partners, including majority ownership interests in Genentech and Chugai, and invested over 8 billion Swiss francs in R&D in 2007. Worldwide, the Group employs about 80,000 people. Additional information is available on the Internet at www.roche.com. NIMBLEGEN, 454, 454 SEQUENCING, and GS FLX TITANIUM are trademarks of Roche. Other brands or product names are trademarks of their respective holders. Contact: Roche Diagnostics GmbH Dr. Burkhard Ziebolz Phone +49 (8856) 60 4830 Email: burkhard.ziebolz@roche.com or Roche NimbleGen Janti Masani Phone +1 608-218-7614 Email: janti.masani@roche.com |
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Copyright ©
2008
Yahoo!7 Pty Limited. All rights reserved.
Advertise with Us -
Privacy Policy - Terms of Service.